Differences in hydrophobic surface characteristics of porcine enteropathogenic Escherichia coli with or without K88 antigen as revealed by hydrophobic interaction chromatography

Hydrophobic Interaction Chromatography

Use of the K88 antigen for in vivo bacterial competition with porcine strains of enteropathogenic Escherichia coli.

Infant mice were used to measure the amount of fluid accumulation (enterosorption) in the intestinal tract after oral inoculation of a porcine strain of enteropathogenic Escherichia coli (K88-+, Ent-+). Significant reduction in the amount of fluid found in the intestinal tract was observed if the mice were first inoculated with a K88-possessing, non-enterotoxigenic strain of E. coli. The protec...

Hydrophobic Interaction Chromatography

modeling for the design of a Biomimetic chimeric ligand. Application to the puriRcation of bovine heart L-lactate dehydrogenase. Biotechnology and Bioengineering 63: 321}331. Lowe CR (1984) Applications of reactive dyes in biotechnology. In: Wiseman A (ed.) Topics in Enzyme and Fermentation Biotechnology, vol. 9. Chichester: Ellis Horwood. Lowe CR, Burton S, Pearson J, Clonis YD and Stead CV (1...

Hydrophobic Interaction Chromatography.

Most proteins and large polypeptides have hydrophobic regions at their surface. These hydrophobic "patches" are due to the presence of the side chains of hydrophobic or nonpolar amino acids such as phenylalanine, tryptophan, alanine, and methionine. These surface hydrophobic regions are interspersed between more hydrophilic or polar regions and the number, size, and distribution of them is a sp...

Escherichia coli K88 Interaction with IgA Oligosaccharides

Diarrhea from enterotoxigenic Escherichia coli (E. coli) expressing the K88 fimbrial adhesin causes high morbidity and mortality among newborn and weaned piglets. K88 fimbrial adhesins are surface filaments with lectin activity that recognize specific glycoconjugates (glycoproteins or glycolipids) on the surface of intestinal cells. Carbohydrates that compete for adhesion attachment could serve...